how to solve keratin
Paul A Dalby
p.dalby at ucl.ac.uk
Wed Sep 13 10:30:39 EST 2000
Immac hair remover contains mercaptoactetate and seems to do the trick.
In article <39BF407D.7ACEE601 at gbf.de>, sandra pilawa <sap at gbf.de> wrote:
>I try to solve keratin (hair of albino rats) to examine the proteins, I
>m looking for a special one. But all I tried (reduction with DTT or
>tributylphosphine and high temperature 95 °C) could not help me to solve
>all the material. Every time a pellet is left.
>So, I m looking for a reducing agent or methode to solve hair totally
>and leave the proteins intact for 2 D electrophoresis.
>thanks for ideas
>Sandra (sap at gbf.de)
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