glycerol gradient

D.Leonard dmarie at gsbs33.gs.uth.tmc.edu
Wed Sep 13 14:52:40 EST 2000


Hi,
I am wanting to do a glycerol gradient to partially purify a 130kDa protein
and to determine if it is present in a complex (by showing up in a higher
sized fraction). I understand this is a older technique, but I haven't ben
able to find a detailed protocol anywhere. Any suggestions or protocols out
there?
TIA,
Deana

-- 
D. Leonard
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