glycerol gradient

Michael Witty mw132 at mole.bio.cam.ac.uk
Sat Sep 16 11:35:48 EST 2000


Did you think of using a freeze thaw method, like for sucrose?  Mike

On Wed, 13 Sep 2000, D.Leonard wrote:

> Hi,
> I am wanting to do a glycerol gradient to partially purify a 130kDa protein
> and to determine if it is present in a complex (by showing up in a higher
> sized fraction). I understand this is a older technique, but I haven't ben
> able to find a detailed protocol anywhere. Any suggestions or protocols out
> there?
> TIA,
> Deana
> 
> -- 
> D. Leonard
> remove a 3 to reply
> 
> 






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