hexahis tags

Michael Witty mw132 at mole.bio.cam.ac.uk
Thu Sep 21 05:45:02 EST 2000

What is the experience of people using hexahis tags for purification?  Do
I need a linker to separate my protein from the tag (like a string of
glycines), or should I just remove the stop codon and add 6 his (or is 10
better?).  Mike.

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