hexahis tags

[Jason McDermott]

We've used a hexahis tag (pet15B) at the N-terminus of a number of proteins
with no problems as far as purification goes. From what I understand, it
seems to be a little protein dependent and if just plugging the his-tag on
doesn't seem to work a good next step would be to introduce a gly linker. I
can't think of a reason C-terminal his-tag wouldn't work.

Jason

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Jason McDermott         |     Anyone can do any amount of work provided
mcdermoj at ohsu.edu       |        it isn't the work he is supposed to be
http://www.jasonya.com  |        doing at the moment. -Robert Benchley
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In article <Pine.SGI.3.96.1000921114251.6211613A-100000 at mole.bio.cam.ac.uk>,
Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:


> What is the experience of people using hexahis tags for purification?  Do
> I need a linker to separate my protein from the tag (like a string of
> glycines), or should I just remove the stop codon and add 6 his (or is 10
> better?).  Mike.
>
>