gelfiltration on superdex 75 pg
Sigrid Van Boxstael
svboxsta at vub.ac.be
Thu Sep 28 10:41:05 EST 2000
I'm trying to purify my protein.
I bring 10 mg (already 90 % pure) on a Pharmacia superdex 75 pg column
I can only recuperate 1.5 mg in the obtained peak.
The buffer I used : Tris 20 mM pH 8.2, 2 mM mercapto-ethanol, 150 mM
When I clean the column after the run there is coming protein of it.
I presume that my protein sticks on the column.
I think that increasing the salt concentrations might be the solution.
Does anybody know either this might help and if yes until what
I can increase it?
More information about the Proteins