gelfiltration on superdex 75 pg
Richard P. Grant
rgrant at netscape.net
Thu Sep 28 11:13:00 EST 2000
In article <39D36691.D21C4C49 at vub.ac.be>, Sigrid Van Boxstael
<svboxsta at vub.ac.be> wrote:
> I can only recuperate 1.5 mg in the obtained peak.
> The buffer I used : Tris 20 mM pH 8.2, 2 mM mercapto-ethanol, 150 mM
> When I clean the column after the run there is coming protein of it.
> I presume that my protein sticks on the column.
Is the column calibrated? Is the 'obtained peak' in the correct place -
are you *sure* it's not running in the void volume? Where do you see
the contaminating protein?
If a fraction of your protein aggregates it could barrel through and
come out first - with you thinking it's the main peak.
Richard P. Grant MAD Phil http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
Please reply to rpg 'at' mrc-lmb.cam.ac.uk'
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