SDS-PAGE running buffer exhaustion
rldurren at bchserver.bch.ncsu.edu
Thu Sep 28 06:47:25 EST 2000
I agree, just make new buffer each time. Maybe start from a 10x stock; it
will last longer.
"Cheryl Whittles" <c.e.whittles at bris.ac.uk> wrote in message
news:39D3299D.5A225510 at bris.ac.uk...
> I don't re-use mine - just make it fresh each time.
> Gerhard Schwall wrote:
> > Does anyone know how to calculate for how long an SDS-PAGE
> > electrophoresis buffer can be re-used. I remember a talk at a conference
> > where the speaker calculated for how long a electrophoresis buffer could
> > be used before exhaustion. Any hints are very much appreciated.
> > Gerhard
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