protein precipitation

kaj.stenberg at noospam.helsinki.fi kaj.stenberg at noospam.helsinki.fi
Fri Sep 29 10:04:19 EST 2000


Suzanne Hermeling <deefsuus at casema.net> wrote:

> I am looking for a protein precipitation method for very low
> concentrated samples (50ug/ml) I have tried chloroform methanol but that
> doesn't work. My samples are in phosphate buffer + n-octylglucoside.
> Does anyboy have an idea?

You do not tell what you intend to do with the protein (and why the
concentration is so low) so I have to suggest freeze-drying. 
   Alternatively, dialyze against high mw PEG or similar, and 
precipitate the concentrate with any method (I prefer ammonium 
sulfate). 

-- 
Kaj






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