extract the cytoskeletal protein

Your name username at hkuxxx.hku.hk
Thu Apr 5 20:47:58 EST 2001


Hi, everybody

I am working on the detecting cytoskeletal protein by 2-D.

During these days, I met the difficulties in the extraction of protein.
I have used the methond based on Moll's methods (Moll R et al. 1982)
But the protein assay show a great little using Bio-Rad DC protein Assay
kit,
whereas I have seen the pellet before last DNase and RNase treation.

Any suggestion is appriecated.

Thanks in advance.

feng

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