finding GFP's mass

Silas Bruun sb at licapharma.com
Sat Apr 7 06:18:50 EST 2001


Hi
How accurate this tool is I don't know. But there must be a dusin of
similar ones you could try also
http://bioinf.mcc.ac.uk/Msc/ysorfd/calc.htm

regards
silas
"Frederic Langlois" <ye.midget.pirates.deleted.my.account at the.seven.seas>
wrote in message news:eppz6.12265$up4.324633 at carnaval.risq.qc.ca...
> Hello.. I'm trying to figure out the different ways to calculate a
protein's
> mass for an assignment. In this particular case I want to know the mass of
> the green fluorescent protein (GFP).
>
> 1) The first method I have used is the SDS-PAGE electrophoresis with the
low
> range standard markers. I made a standard curve of the standards Rf value
(Y
> axis) and the molecular weight (X axis). I extrapolated the GFP's mass
using
> it's Rf value.
>
> 2) Then I searched many databanks and found GFP's amino acid sequence. I
> used this equation to find GFP's mass:
>
> mass = [(n1*molecular
>
weight1)+(n2*mol.weight2)+...(n20*mol.weight20)]/[(n1+n2+...n20-1)*33g/mol]
>
> where 1=glycin; 2 to 20= all the other amino acids
>       n=the sum of the amino acids of each kind
>       33g/mol=a water molecule's molecular weight (approximately)
>
> I substrated the water molecules that are "lost" when the peptidic bonds
are
> made.
>
> I converted the molecular weight in Dalton units...
>
> 3) what are the other methods I can use to find the mass of this protein?
>
> Thanks in advance,
>
> Frederic Langlois
>
>
>





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