Ammonium Sulfate

Dr. Artem Evdokimov eudokima at mail.ncifcrf.gov
Tue Aug 7 18:42:53 EST 2001


And if you intend to crystallize the protein, don't do this. Even
ammonium sulphate precipitation may not be safe, in fact.

A.

Dima Klenchin wrote:
> 
> "Marie France  Janelle" <mariefrancejanelle at sympatico.ca> wrote:
> 
> >I used ammonium sulfate to precipite a little protein (12 kDa). I want to
> >get rid of ammonium sulfate now. Is someone have a method to do that
> >successfully?
> 
> To the already mentioned in other replies I can add this:
> 
> Usially 12K proteins are quite simple and withstand quite harch
> treatments. If yours is such a case, an attractive possibility can be
> organic solvent precipitation. Say, you have your pellet after AmSO4
> cut, you resuspend it in water/buffer, then add enough acetone
> (isopropanol, dioxane or whatever works best keeping protein alive),
> your protein precipitates and you resuspend it in whatever you like.
> The advantage to dialysis is speed (fast) and keeping concentration
> high. Works only for select proteins, _usually_ small.
> 
>         - Dima

-- 
|Dr. Artem Evdokimov   Protein Engineering |
| NCI-Frederick        Tel. (301)846-5401  |
|              FAX (301)846-7148           |
|        eudokima at mail.ncifcrf.gov         |
|      http://mcl1.ncifcrf.gov/plague      |




More information about the Proteins mailing list