Ammonium Sulfate

Frank Fürst ffrank at
Wed Aug 8 06:48:51 EST 2001

klenchin at (Dima Klenchin) wrote:

> P.S. A question: protein from inclusion bodies spontaneopusly 
> misfolds into something 100% inactive but 100% soluble. What is the 
> best way to see if resulting sup is homogenous or not? More 
> filosophical Q: what are the chances of this conformation being 
> relevant to anything? (In other words, did Nature missed it or went
> for a trouble of using alternative that does not fold spontaneously?)

Might be relevant. E.G. some "cofactor" might be missing, which can be a
protein that is needed for correct refolding (PPIases, PDI's) or
assembly. Are you sure about the identity and size of the native
protein? Might be a heterodimer, and only one of them is in the IB, or
other strange things, like a pro-sequence inhibiting activity.

Bye, Frank

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