OD and IPTG concentration
junk at 127.0.0.1
Thu Dec 20 07:01:22 EST 2001
In message <45249c2f.0112161713.578808c4 at posting.google.com>, S.Ballal
<ballal_sv at hotmail.com> writes on the Sun, 16 Dec 2001
>I would like to know how is the optimum concentration of IPTG used for
>induction related to OD of the culture.
>In most of the places varying concentration of IPTG has been suggested
>for induction depending upon the expression system and other things.
>Most of these are for an low ODs like 0.5-1.
>In case we need to work on high ODs like 50-100 or 150 would one
>require much higher concentration of IPTG and if in what range is
>generally used for such cultures.
I've always used the same concentration regardless of OD.
The more critical bit is stage of growth rather than OD itself i.e.
culture must be in logarithmic growth and not late log or stationary.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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