homodimers

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Thu Dec 20 00:06:23 EST 2001


1) Separate the complex, run an SDS gel or obtain an MS spectrum. If you
have an unknown protein, there will be an unknown mass out there.

A.G.E.

"Mathew Guilfoyle" <GuilfoyleMR at cardiff.ac.uk> wrote in message
news:4b9ea6a.0112190530.52ced746 at posting.google.com...
> Does know of a (relatively) simple method for determining whether a
> protein is a homodimer or not? Obviously a western will indicate an Mr
> of double the monomer, but how do you demonstrate homodimerization
> rather than heterodimerization to an unknown protein?
>
> cheers





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