SDS-PAGE of low pI proteins

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Mon Dec 24 12:36:44 EST 2001


In my humble experience, if I noticed any abnormalities they were in the
other direction - i.e. they usually migrated faster for me. But then, the
lowest protein pI  I have worked with was about 2.3.

Artem

A.G.E.
"Kristina Warton" <K.Warton at cfi.unsw.edu.au> wrote in message
news:20011223061900.25319.qmail at ww02.jatek.com...
> Is anyone aware of published work showing that proteins with very low
> isoelectric points migrate aberrantly slowly on SDS-PAGE? I think it may
be
> because they bind less SDS and end up with a lower overall negative
charge.
> I've seen this glossed over in a paper, but I haven't been able to find
any
> methods papers where this is specifically looked at.
>
> Kristina
>
>
> <http://www.biowww.net/forum/read.php?f=6&i=882&t=882>
>





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