Post translational modification??
sergioal at bbm1.ucm.es
Fri Feb 2 05:34:56 EST 2001
proteolysis? maybe that aa is within a peptidase recognition site.
> Hi all,
> I am studying the expression of two highly homologous proteins
> (approximately 50 kDa). In fact their published sequence differs by
> only ONE amino acid. Using a monoclonal antibody that detects BOTH
> proteins, I can resolve the two proteins by SDS page as if there is a
> 5kDa difference, when in fact there is only one amino acid difference.
> At this time, there is no evidence for phosphorylation differences or
> glycosylation differences.
> Does anyone have any ideas as to why I can see such a big difference
> by SDS PAGE even thought the proteins are only 1 amino acid difference
> in length?
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