trypsin digestion and structure of protein

Michael Witty mw132 at mole.bio.cam.ac.uk
Mon Feb 5 05:34:37 EST 2001


My boss says that if I can digest my protein with trypsin and discrete
bands appear, rather than total digestion, I can conclude it has some
structure other than random coil.

Well, my protein is digested to nothing, compared to BSA, which is hardly
touched.

Do we all think that my protein has no ordered structure in solution, and
is therefore not worth crystallizing?  Mike.






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