Gel voltages and times
Paul S. Brookes.
brookes at uab.edu
Fri Feb 9 09:05:42 EST 2001
I have a real silly question about gel voltages.... Is the voltage you
apply across them determined by the length, or the cross-sectional area?
The protocol I'm working from uses 120V, 12hrs, for a 140 x 140 x 1.5mm
gel - i.e. 210square mm cross-sectional area, 140mm length. I have a 70
x 90 x 1.5mm mini-gel, i.e. 135 square mm area, 70mm length.
* So, seeing as my gel's x-sectional area is 64% of the bigger one, do I
use 64% of 120V = 77V
* Or because the length is 50% do I use 50% of 120V = 60V
* And how long to run for? Does half the length = run for half the time?
Is there a simple equation for working out voltages based on gel % and
Thanks in advence for any help.
Dr. Paul S. Brookes. (brookes at uab.edu)
UAB Department of Pathology, G004 Volker Hall
1670 University Blvd., Birmingham AL 35294 USA
Tel (001) 205 934 1915 Fax (001) 205 934 1775
The quality of e-mails can go down as well as up
-------------- next part --------------
An HTML attachment was scrubbed...
More information about the Proteins