Low protein amount - what to do?

Silas Bruun sb at licapharma.com
Wed Feb 28 10:13:55 EST 2001

I have cloned a gene in the "in vitrogen" pBAD and expressed it in the TOP10
Coli cells that comes along with the kit. But I really don't get much
protein (very litle) out of it.

Do anyone know how to boost these cells or know any other Coli strains that
might work with this vector and which give alot op protein (strain should be


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