His-tags and DNA-binding

Matt Hicks matth at biols.susx.ac.uk
Thu Jan 11 05:51:29 EST 2001


Hi Folks,
Has anyone come across a situation where a 6XHis tag has affected the
DNA-binding of a DNA-binding protein? (the pH is 8 so unlikely to be +ve
charge). I know the folding could be affected. Just wondered if anyone
had seen such a phenomenon...
Thanks,

**************************************************

Dr. Matt Hicks
School of Biological Sciences
University of Sussex
Falmer
BN1 9QG
UK      

Tel:   +44(0)1273 678923
Fax:   +44(0)1273 678433
email: matth at biols.sussex.ac.uk                                                  

**************************************************






More information about the Proteins mailing list