wgallin at gpu.srv.ualberta.ca
Mon Jan 15 16:54:44 EST 2001
FITC stands for fluorescein isothiocyanate, a reagent that is commonly
used to derivatize proteins with a fluorescein group (often an antibody,
sometimes other proteins like lectins). Fluorescein fluoresces a kind
of apple green when excited with near UV light.
This is a fairly easy way of detecting the presence of a given ligand
for the derivatized protein at the light microscopic level of
resolution. You need a microscope that has an optical setup that will
shine excitatory wavelength onto the field of view and a set of filters
and/or dichroic mirrors that will pass only the fluorescent light up
through the optical path leading to the eyepieces. Microscopes like
this are commercially available.
Frank Fuerst wrote:
> bmolecular at uclv.etecsa.cu ("bmolecular") wrote:
> >Dear All,
> >I will thank very much if someone can give any information about FITC fluorescence microscopy and the principle of function of this technique.
> >Thanks in advance,
> Uups, what does that abbreviation mean? Fluorescence intensity
> Entschuldigung wegen dem schlechten Posting, aber
> ich denke jeder ist mal angefangen.
> [N.N in dcoulmisc]
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