Western blotting from Blue Native gel

Nora Plesofsky nora at biosci.cbs.umn.edu
Thu Jan 18 17:42:13 EST 2001


I am planning to run a Blue Native gel, followed by electroblotting 
for Western analysis. I have read that the Coomassie blue that binds 
proteins in Blue Native  electrophoresis interferes with protein 
transfer to a membrane. Yet, there are many reports of transfer from 
BN gels.

Does anyone have experience with this procedure who could give me 
some tips? And is it possible that a nitrocellulose membrane presents 
more of a problem than PVDF?

Thanks for any help.

Nora Plesofsky


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