Western blotting from Blue Native gel
nora at biosci.cbs.umn.edu
Thu Jan 18 17:42:13 EST 2001
I am planning to run a Blue Native gel, followed by electroblotting
for Western analysis. I have read that the Coomassie blue that binds
proteins in Blue Native electrophoresis interferes with protein
transfer to a membrane. Yet, there are many reports of transfer from
Does anyone have experience with this procedure who could give me
some tips? And is it possible that a nitrocellulose membrane presents
more of a problem than PVDF?
Thanks for any help.
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