gentle elution buffer

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Fri Jan 19 10:02:50 EST 2001


In article <de1949.fe7.ln at wpxx02.toxi.uni-wuerzburg.de>,
  Cornelius Krasel <krasel at wpxx02.toxi.uni-wuerzburg.de> wrote:
> Dima Klenchin <klenchin at remove_to_reply.facstaff.wisc.edu> wrote:
> > P.S. Isn't it funny that Pierce recommends storing this solution
> > in the cold? Like anything can grow in 3.5 M MgCl2!
>
> Halobacteria?
>
> I have seen a lot of funny organisms (mostly fungi) growing in solutions
> where I never would have expected it. The most impressive thing that I
> can remember off-hand is some molds growing in 10% SDS.
>

Wow, that sure beats the mold or fungus (something fuzzy, anyway, and it
was degrading my polysomes) I found growing in 10% NP-40 in the cold, and
people wonder why I now keep it frozen.

Nick


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Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu


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