Binding insoluble protein to Affi-gel

Dr. Artem Evdokimov eudokima at mail.ncifcrf.gov
Tue Jul 17 16:06:55 EST 2001


What about guanidine hydrochloride ? As a salt, the guanidinium group is
very unreactive. Have you considered detergents (Tween, Triton, etc) or
perhaps nondetergent sulphobetaines ?

A.

Lee Hunt wrote:
> 
> I've purified a His- tagged protein from inclusion bodies using 6M urea.
> How can I then link it to affi-gel?. I've thought of dialysing but would
> prefer there to be a denaturant that doesn't have primary amine groups
> to replace the urea

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|Dr. Artem Evdokimov   Protein Engineering |
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