SDS-PAGE running problem, help!

Amanda Cage cage_amanda at hotmail.com
Wed Jul 18 00:05:43 EST 2001


I have problems running SDS-PAGE gels because a pencil-like line runs
continuously from one end of the gel to the other end of the gel
horizontally at about 3/4 of the way down on the gel when I do the
silver staining.  I am not sure why that happens and do not know how
to get rid of it.  The strange thing is when I use commassie blue to
stain a different gel (not the same gel as silver but contain the same
samples), a color gradient appear across the gel.  The top appear
almost colorless.  The middle of the gel appears a light blue.  The
bottom part of the gel (3/4 way down) appears a deeper blue than the
middle.  I could not get the entire gel to stain a uniform blue color.
 Could the problems with these two gels be the running buffer
contaminants or is it something else?  I washed the SDS off the gel
very well so I believe that is not the problem.  Please help!  Thank
you.




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