Western blues

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Fri Jun 22 23:25:08 EST 2001


In <JFEILCKKLMIGLOJJOONCKECBCAAA.tdschr0 at pop.uky.edu>, Todd Schraw wrote:
>
[...]
>
>In several experiments I have seen better transfer of higher molecular
>weight proteins with the addition of SDS. Also we have had problems with
>poor grades of Tris base that give acidic pH once dissolved in water instead
>of the usual ~8.5.

Well, that would happen if you used TrisHCl instead of Tris base.

>Another aspect that they repeatidly point to is the possiblity of a short by
>using nitrocelluse or stack paper that is larger than the gel itself. The
>make a big deal about having these exaclty the same size as the gel.
>

You must be joking (or they are).

Nick

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu

 




More information about the Proteins mailing list