Cloning efficiency vs. vector size.

Tomas Aparicio tomas at solea.quim.ucm.es
Thu Mar 1 03:31:18 EST 2001


Good morning/evening:

    I have a basic doubt about cloning eficiency depending upon vector size:

    Using the same replication origin (for instance, a ColE1 derived
vector), is important the vector size in order to clone very big inserts?
    And another question: have anybody succeeded in cloning PCR fragments
bigger than 17 Kb? Which vector did you use?

Thanks in advance, and good luck.

Tomas Aparicio.







More information about the Proteins mailing list