Cloning efficiency vs. vector size.

Tomas Aparicio tomas at
Thu Mar 1 03:31:18 EST 2001

Good morning/evening:

    I have a basic doubt about cloning eficiency depending upon vector size:

    Using the same replication origin (for instance, a ColE1 derived
vector), is important the vector size in order to clone very big inserts?
    And another question: have anybody succeeded in cloning PCR fragments
bigger than 17 Kb? Which vector did you use?

Thanks in advance, and good luck.

Tomas Aparicio.

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