GST-fusion problems

Dr. Duncan Clark d_clark at
Mon Mar 5 07:36:33 EST 2001

In article <3AA37B33.B1AF72B0 at>, the eminent
Kevin Brady at Organic Chemistry, Nottingham wrote
>    When I run fractions out on an SDS-PAGE gel, I can see the 37kDa 
>    band, but also about 6 smaller proteins ranging from about 
>    35-22kDa. These dont appear in the wash steps at all, and are 
>    specifically eluted with GSH. I'm using BL21(DE3)pLysS E.coli with 
>    pET42b for expression. Has any one else seen these sorts of 
>    proteins appearing? 

Proteolysis of the fusion during expression?

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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