GST-fusion problems

Dr. Duncan Clark d_clark at nospam.demon.co.uk
Mon Mar 5 07:36:33 EST 2001


In article <3AA37B33.B1AF72B0 at unix.ccc.nottingham.ac.uk>, the eminent
Kevin Brady at Organic Chemistry, Nottingham wrote
>    When I run fractions out on an SDS-PAGE gel, I can see the 37kDa 
>    band, but also about 6 smaller proteins ranging from about 
>    35-22kDa. These dont appear in the wash steps at all, and are 
>    specifically eluted with GSH. I'm using BL21(DE3)pLysS E.coli with 
>    pET42b for expression. Has any one else seen these sorts of 
>    proteins appearing? 

Proteolysis of the fusion during expression?

Duncan
-- 
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sliced from time to time....

Duncan Clark
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