Dr. Duncan Clark
d_clark at nospam.demon.co.uk
Mon Mar 5 07:36:33 EST 2001
In article <3AA37B33.B1AF72B0 at unix.ccc.nottingham.ac.uk>, the eminent
Kevin Brady at Organic Chemistry, Nottingham wrote
> When I run fractions out on an SDS-PAGE gel, I can see the 37kDa
> band, but also about 6 smaller proteins ranging from about
> 35-22kDa. These dont appear in the wash steps at all, and are
> specifically eluted with GSH. I'm using BL21(DE3)pLysS E.coli with
> pET42b for expression. Has any one else seen these sorts of
> proteins appearing?
Proteolysis of the fusion during expression?
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
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