GST-fusion problems

Dr. Duncan Clark d_clark at
Mon Mar 5 10:25:46 EST 2001

In article <3AA3A05E.670AE87E at>, the eminent
Kevin Brady at Organic Chemistry, Nottingham wrote
>    Duncan, which protease inhibitor(s) would you recommend if it is 
>    proteolysis, AEBSF..? 

How long is a piece of string?

AEBSF is a start but I think there are some multiple-inhibitor cocktails
available from Roche Diagnostics. I've been very fortunate and not had
proteolysis problems, probably something to do with expressing
thermophilic proteins and lysing E.coli at 80C!

If you do buy from Roche remember they have a three for the price of two
day in the UK on 21st March! 

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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