GST-fusion problems

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Mon Mar 5 12:30:50 EST 2001


Kevin Brady <rcxkb at unix.ccc.nottingham.ac.uk> wrote:
:
:Duncan, which protease inhibitor(s) would you recommend if it is
:proteolysis, AEBSF..?

Kevin, inhibitors would only help if your proteolysis 
occurs post-lysis. If it happens during expression
(inside bugs) - this could be both premature termination
or true proteolysis - then there is not much you 
can do. 

        - Dima






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