Fusion proteins - How's it done?

terry morris tmorris at uhnres.utoronto.ca
Wed Mar 7 16:35:58 EST 2001


Hi Anyone who knows,

Can somone tell me how fusion gene constructs are made i.e. say I wanted to
link a gene to GST or GFP tags(suppose I've got cDNA all for these). How do
I glue the two strands of DNA together before I can express it to produce a
single fusion protein.

Thanks,

Terry










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