Fusion proteins - How's it done?

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Thu Mar 8 17:59:14 EST 2001


"terry morris" <tmorris at uhnres.utoronto.ca> wrote:
:Hi Anyone who knows,
:
:Can somone tell me how fusion gene constructs are made i.e. say I wanted to
:link a gene to GST or GFP tags(suppose I've got cDNA all for these). How do
:I glue the two strands of DNA together before I can express it to produce a
:single fusion protein.

To "glue" two genes to produce a fusion product, you
ligate two cDNAs in frame.

        - Dima







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