His tag.

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Thu Mar 15 12:38:39 EST 2001


skumar at mbu.iisc.ernet.in ("S. Kumar Singh") wrote:
:Hi all,
:Is there any good established procedure for removing His tag from the N
:terminus of a protein., I would greatly appreciate a reference on this.
:Thanks a million in advance.

Only if you have protease recognition site between the tag and
the protein. Most conventional proteases are not specific enough
and cut expressed protein as well, however. TEV protease and
its 6 aa recognition site appears to be the best choice. Look up
Gibco catalog for more details.

        - Dima




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