Crude enzyme preparations

Dana Summers dsummers at mtcnet.net
Thu Mar 15 18:13:26 EST 2001


I am enrolled in a enzyme/biochemistry class at Northwestern College.
Each student drew from a box various enzymes that are currently
manufactured and is required to research that enzyme (where it comes
from, what it is used for, how to test its activity, how it is
isolated/manufactured etc.)  I have been paired up with two other
students one who is researching phospholipase A2, the other chymotrypsin
and for myself I have trypsin. All three of us have obtained the
procedures on how to assay the 3 enzymes and  have obtained commercial
samples of each enzyme and have performed the assays with good success.
The three of us have yet to find any methods in the manufacturing of
these enzymes ( hopefully from swine pancreas since we have access to
this tissue) to make our own crude preparations of these enzymes so that
we can test their activity. Any help would be greatly appreciated by the
three of us since the semester is coming to a close very soon. Kevin

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