pH inside E.coli?

Nick Theodorakis nicholas_theodorakis at
Sat Mar 17 21:50:03 EST 2001

In <990u9f$g0g$1 at>, Jeffery Haines wrote:
>:I'm not 100% sure, but I assuming it is around 7.2 physiological pH to 
>maintain structural stability of proteins/enzymes etc.  I know when we 
>make up LB broth it is pH 7.2-7.4 and the bugs are very happy growing in 

LB isn't buffered, so it's pH probably ends up being whatever the bugs decide to
make it.

ISTR that bacteria maintain an electrochemical gradient across the membrane, so
the pH inside doesn't have to be the same as the outside pH.


Nick Theodorakis
nicholas_theodorakis at

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