his tag

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Wed Mar 21 09:19:45 EST 2001


Michael Witty <mw132 at mole.bio.cam.ac.uk> wrote:
> Are there examples of proteins that people have added internal his tags too?
> For example on an exposed loop?

Yes; for example there is a G-protein alpha subunit construct made by
Christiane Kleuss which has 6 His fused in-frame into an exposed loop
on the helical domain of the alpha subunit. To enhance flexibility of
the His-tag, it is framed by two Gly residues at each side.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: krasel at wpxx02.toxi.uni-wuerzburg.de */
/* "Science is the game we play with God to find out what His rules are."  */




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