His-tagged proteins....

Michael Witty mw132 at mole.bio.cam.ac.uk
Mon Mar 26 04:04:43 EST 2001


On Sun, 25 Mar 2001, ChenHA wrote:
> Frank Fuerst wrote:
> > But the hist tag might well interfere with crystallization (it might
> > even improve it...).
> There are anecdotal evidence that suggest that the his tag may help
> improve crystallization (and you won't see the his-tag in the
> crystal).  Therefore nothing should be removed unless there are
> reasons to do so.

Dear Chen,
         I agree.  But could you tell me if there are any pdb files you
know of that DO have the coordinates of a his tag (maybe with Ni in
place).  It would be interesting to be able to display one.  Mike.




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