His-tagged proteins....

Simon Holton simon at biop.ox.ac.uk
Mon Mar 26 05:02:22 EST 2001


Can you expand a little on the anecdotal evidence you mention?

Thanks

Simon

> From: ChenHA <hzhen at freeuk.com>
> Newsgroups: bionet.molbio.proteins
> Date: Sun, 25 Mar 2001 17:20:43 +0100
> Subject: Re: His-tagged proteins....
> 
> 
> 
> Frank Fuerst wrote:
>> 
>> 
>> But the hist tag might well interfere with crystallization (it might
>> even improve it...).
>> 
> 
> There are anecdotal evidence that suggest that the his tag may help
> improve crystallization (and you won't see the his-tag in the
> crystal).  Therefore nothing should be removed unless there are
> reasons to do so.




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