imidazole

Dr. Artem Evdokimov eudokima at mail.ncifcrf.gov
Tue May 1 09:58:36 EST 2001


> But the happy ending is that I eventually got 0.52mm long crystals.  And
> you are right, the plasticware I used was full of dust!  Mike.

Congrats !

What really bites is that only about 6-15% of all proteins that are
cloned more or less indiscriminately (as in 'structural genomics') ever
crystallize. We have a sample of about 40 proteins but don't take my
word for it - recent results of the structural genomics consortia show
the same trend.

We have a pretty good methodology to bring the proteins that show
crystallinity to the final stage (i.e. well behaved single crystals) but
the problem is still getting this first hit.

Artem

-- 
|Dr. Artem Evdokimov   Protein Engineering |
| NCI-Frederick        Tel. (301)846-5401  |
|              FAX (301)846-7148           |
|        eudokima at mail.ncifcrf.gov         |
|      http://www.ncifcrf.gov/plague       |




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