Talon resin histidine use

Dr. Artem Evdokimov eudokima at mail.ncifcrf.gov
Fri May 4 07:24:40 EST 2001


It should work however the question of protein stability in these
conditions is still there.

I would try EDTA first, unless you know that your protein contains
structurally vital metal ions.

Also, why can you try Ni-NTA ? It has higher affinity for Ni ions and
might not be leached out by histidine.

A.

Jeremy Murray wrote:
> 
> What about low pH or EDTA?
> 
> John Eisses wrote:
> 
> > Bionetters,
> > I am purifying a delicate membrane protein and imidazole seems to
> > inhibit it, but when I use histidine, the Talon (Co2+) resin bleaches
> > out and the buffer turns brown. Are there alternatives for imidazole? I
> > seem to remember a ref for N acetyl Histidine , but does this strip the
> > Talon resin? I'll try buffer exchange after the fact but I'd like to not
> > lose activity as my experience is once its gone it doesn't come back.
> >
> > salud
> > Ted Michelini
> > michelini at ohsu.edu

-- 
|Dr. Artem Evdokimov   Protein Engineering |
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