How to renature His-tagged protein

Bull Zebra bullzebra at
Tue May 8 16:47:42 EST 2001

I am purifying a 6XHis-tagged protein under denature condition (8M urea)
using Ni-NTA agarose and I need to renature the protein in order to couple
it to an affinity column (CNBr method). Anybody there can suggest a good
renaturation protocol for this purpose? Thanks in advance!

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