folding proteins

Inés Baeza Hernández ibh at ono.com
Fri May 11 14:25:04 EST 2001


I'm working in overexpression of an halophilic protein in E.coli. I obtain
my protein as inclusion boides, but I cannot refolding this. I have proven
with some buffers (Tris, Hepes, phosphate) at different pH, temperature and
salt concentration.

Have you some idea? Thanks in advance!







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