Connoisseurs of Ni-NTA

Michael Witty mw132 at mole.bio.cam.ac.uk
Mon May 14 09:27:58 EST 2001


Here is one for connoisseurs of Ni-NTA.  When I loaded a protein (22kDa)
onto Ni-NTA, I eluted, roughly, 22, 44, 66kDa.  This protein has
disulphide bonds and I suspect some kind of bad breaking of disulphide
bonds and reforming between molecules.  Does anyone have similar
experience of this kind of thing?  Mike.




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