Electrotransfer proteins for Edman sequencing
khatipovNO at NOuchicago.edu
Wed Nov 7 12:56:30 EST 2001
I am always sloppy in maths, trying not to be in everything else:-) Thanks
for your correction. In fact, I was instructed by the facility staff to load
at least 1-2ug of protein anyway.
"D.K." <dk at no.email.thankstospam.net> wrote in message
news:9sbifn$135o$1 at news.doit.wisc.edu...
> "Emir Khatipov" <khatipovNO at NOuchicago.edu> wrote:
> >I was a bit irritated by the
> >abundance of vague or sometime condescending phrasing in your message
> >you don't have [a lot of protein] ..., save yourself time and send it to
> >mass-spec-based sequencing"
> Condescending? Hmm. Whatever - as long as it helps to find 50-fold mistake
> in your calculations.
> >- which we don't have in our university;
> Many facilities in other universities accept samples. Harvard
> Microsequencing facility is outstanding (long waits and expense are
> drawbacks), if you can't wait, Yale and Michigan usually do very
> good job as well. I am sure there are many others.
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