6His co-eluted protein ?

D.K. dk at no.email.thankstospam.net
Thu Nov 8 20:05:46 EST 2001


"Emir Khatipov" <khatipovNO at NOuchicago.edu> wrote:
>Hi All,
>Someone recently posted here that the E.coli protein that many co-purify on
>a Ni or Co columns is actually a proline ...-ase. I failed to find this
>message in archives, and I would appreciate someone re-posting which enzyme
>that was and what is its MW. My colleague has doublets coeluted from
>Co-column (only one of them gives a specific band on Western - ~48kDa) and I
>wonder if she copurifying that proline enzyme.

Nope, that "...ase" is proline isomerase, which is a ~21 kDa protein.

There are few cases where mammalian protein would bind an E.coli homologue 
of its physiological interactor... if the band is strong, persistent and "specific",
it is something to think about. 

DK







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