Antibody recognition of a protease created epitope
osullivan at mpih-frankfurt.mpg.de
Sun Nov 25 14:22:28 EST 2001
I am trying to identify a protein after cleavage by a protease and follow it as oppsed to the intact version.
One way would be to use a commercially available antibody that will recognise a defined set of residues only after proteolytic cleavage and not in the uncleaved protein. Thus, cleavage of the peptide bond will leave a new N-terminus followed by a series of residues which will create a new epitope not present in the intact protein. Does anybody know of such an antibody ?
Alternatively, perhaps is there an 'in vivo', non-toxic way of labelling an amino acid (e.g. lysine) with a free N-terminus ?
Any suggestions will be greatly appreciated.
Gregory A. O'Sullivan PhD.
Dept. of Neurochemistry,
Max Planck Institut fur Hirnfornschung,
Tel: +49 69 96769222
Email: osullivan at mpih-frankfurt.mpg.de
-------------- next part --------------
An HTML attachment was scrubbed...
More information about the Proteins