Over-expression using maltose-binding protein
arsenio.villarejo at plantphys.umu.se
Thu Oct 4 11:57:39 EST 2001
I'm trying to over-express a protein using the
maltose-binding protein system. My protein is quite hydrophobic and
has an isoelectric point close to 9.5. After cutting the fusion
protein with Xa factor, I tried to purify the cleaved protein by ion
exchange chromatography. At this point, I lost my protein because it
binds to everything (dialisis membranes, centricon membranes,....).
So, I should keep a relative high salt concentration in the mixture
to avoid any precipitation of my protein.
What kind of chromatography technique can I used for
separating the maltose binding protein from my protein (with ca. 30
Any help or new idea will be wellcome.
Thanks a lot in advance.
Arsenio Villarejo Phone: +46 90 7867648
Department of Plant Physiology Fax: +46 90 7866676
S-901 87 Umea, Sweden E-mail: Arsenio.villarejo at plantphys.umu.se
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