Immunohistochemistry - Leakage of protein from nucleus?

Cornelius Krasel krasel at
Fri Oct 5 08:03:31 EST 2001

In bionet.molbio.methds-reagnts Emir Khatipov <khatipovNO at> wrote:
> I recommend paraformaldehyde (PFA) fixation:
> 3% PFA/2% sucrose in PBS for 5 min at R/T, and wash with PBS 2-3 times.

Stupid question: why do some people add sucrose to their paraformaldehyde


/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: krasel at */
/* "Science is the game we play with God to find out what His rules are."  */

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