Immunohistochemistry - Leakage of protein from nucleus?
krasel at wpxx02.toxi.uni-wuerzburg.de
Fri Oct 5 08:03:31 EST 2001
In bionet.molbio.methds-reagnts Emir Khatipov <khatipovNO at nouchicago.edu> wrote:
> I recommend paraformaldehyde (PFA) fixation:
> 3% PFA/2% sucrose in PBS for 5 min at R/T, and wash with PBS 2-3 times.
Stupid question: why do some people add sucrose to their paraformaldehyde
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany email: krasel at wpxx02.toxi.uni-wuerzburg.de */
/* "Science is the game we play with God to find out what His rules are." */
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