his-tagged protein contaminate

Lera lera at ut.ee
Fri Oct 5 10:42:07 EST 2001


Hello!
I purified 27kDa His-tagged protein (expressed in E. coli) on Ni-NTA
column, 80kDa contaminate protein always presents in eluates. Could
anybody recommend me some procedures to avoid the presence of this 80kDa
protein? Thanks!!!
Valeria.







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